Part:BBa_K5403007

N-terminal transmembrane domain of PorinMspA

Porin MspA, see figure 1, is an ion channel protein that is found in m. smegmatis extracellular vesicles (Prados-Rosales et al., 2011).

Figure 1: Structure of porin MspA (M. smegmatis) (PDB '1UUN', 2004).

This part was designed as a domain for the composite part BBa_K5403016, which is a fusion protein of the signal sequence of porinMspA, a flexible linker and GFP, aiming to anchor the GFP into the membrane of the vesicles of M. smegmatis.

References

Prados-Rosales, R., Baena, A., Martinez, L. R., Luque-Garcia, J., Kalscheuer, R., Veeraraghavan, U., Camara, C., Nosanchuk, J. D., Besra, G. S., Chen, B., Jimenez, J., Glatman-Freedman, A., Jacobs, W. R., Porcelli, S. A., & Casadevall, A. (2011). Mycobacteria release active membrane vesicles that modulate immune responses in a TLR2-dependent manner in mice. Journal Of Clinical Investigation, 121(4), 1471–1483. https://doi.org/10.1172/jci44261 RCSB (2004) PDB - 1UUN: Main porin from Mycobacterium smegmatis (MspA). https://www.rcsb.org/structure/1UUN

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]